spontaneous fluorescence quencher solution b  (Servicebio Inc)

Journal: Chinese Medicine

Article Title: Targeting the ferritinophagy axis: multi-target regulation of TFRC/FTH1/NCOA4 by artesunate ameliorates salivary gland dysfunction in Sjögren’s Disease

doi: 10.1186/s13020-026-01354-8

Figure Lengend Snippet: ART mitigates salivary gland dysfunction in SJD model mice. A Histological assessment: H&E staining of submandibular gland sections from different groups. Black boxed regions are shown at ×10 magnification in adjacent panels. Original magnification: ×40; scale bar: 2.5 mm. B Quantification of the inflammatory area (%) in salivary glands (SGs; n = 10). C Salivary gland index (salivary gland weight/body weight) in the indicated groups ( n = 10). D Plasma IgG levels in the indicated groups ( n = 10). E Average body weight of NOD mice under different treatments. Plasma levels of creatinine (Cr; F ), alanine transaminase (ALT; G ), and aspartate transaminase (AST; H ) in the indicated groups ( n = 10 per group). I – K Confocal microscopy detection of IgG and C3 deposition in submandibular glands under different treatments. Scale bar = 150 μm. Semiquantitative analysis of IgG and C3 fluorescence intensity was performed using ImageJ software (20 images from 3 mice/group, n = 3). Data are mean ± SEM.* p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Briefly, the sections were subjected to antigen retrieval and treated with a tissue spontaneous fluorescence quencher (G1221, Servicebio).

Techniques: Staining, Clinical Proteomics, Confocal Microscopy, Fluorescence, Software

Journal: Chinese Medicine

Article Title: Targeting the ferritinophagy axis: multi-target regulation of TFRC/FTH1/NCOA4 by artesunate ameliorates salivary gland dysfunction in Sjögren’s Disease

doi: 10.1186/s13020-026-01354-8

Figure Lengend Snippet: Artemisinin derivative restores salivary gland homeostasis through a non-apoptosis-inhibitory mechanism. A Average water intake of ICR and NOD mice (in vivo). B Saliva flow rate (SFR, n = 10) in mice (in vivo). C CCK-8 assay for SGEC viability (human A253 cells, in vitro, n = 4). D Western blot and quantification of AQP5 in mice (in vivo, n = 3). E Western blot and quantification of AQP5 in SGECs (human A253 cells, in vitro, n = 3). F , G Confocal images of AQP5 (scale bar = 150 μm) with semi-quantification of relative mean fluorescence intensity (MFI) 20 images from 3 mice/group (in vivo, n = 3). H Cell apoptosis assessed by flow cytometry (human A253 cells, in vitro, n = 3). Data are mean ± SEM.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Briefly, the sections were subjected to antigen retrieval and treated with a tissue spontaneous fluorescence quencher (G1221, Servicebio).

Techniques: In Vivo, CCK-8 Assay, In Vitro, Western Blot, Fluorescence, Flow Cytometry

Journal: Chinese Medicine

Article Title: Targeting the ferritinophagy axis: multi-target regulation of TFRC/FTH1/NCOA4 by artesunate ameliorates salivary gland dysfunction in Sjögren’s Disease

doi: 10.1186/s13020-026-01354-8

Figure Lengend Snippet: Artesunate suppresses ferroptosis by modulating lipid peroxidation and redox homeostasis. A , B Western blot analysis and quantification of ACSL4 and GPX4. C – F Confocal microscopy of NRF2, GPX4, and ACSL4 localization in submandibular glands, with semi-quantification of relative mean fluorescence intensity (MFI; 20 images from 3 mice/group, n = 3). Scale = 150 μm/100 μm. G , H Western blot analysis and quantification of xCT. I , J Confocal microscopy of AQP5 and xCT localization in submandibular glands, with semi-quantification of MFI (20 images from 3 mice/group, n = 3). Scale = 150 μm. K , L GSH and MDA levels in salivary gland tissues ( n = 10). O Prussian blue staining (blue) indicates Fe 3+ accumulation. Black boxed regions are shown at ×30 magnification in adjacent panels; original magnification: ×20, scale bar: 4500 μm. Black arrows point to Fe 3+ accumulation in salivary gland tissues ( n = 10). P Relative Fe 3+ accumulation is expressed as the percentage of Prussian blue-positive staining phenotype. 2D intensity histograms show Pearson’s coefficient (PR), red Manders’ coefficient (M1), and green Manders’ coefficient (M2). Data are mean ± SEM.* p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Briefly, the sections were subjected to antigen retrieval and treated with a tissue spontaneous fluorescence quencher (G1221, Servicebio).

Techniques: Western Blot, Confocal Microscopy, Fluorescence, Staining

Journal: Chinese Medicine

Article Title: Targeting the ferritinophagy axis: multi-target regulation of TFRC/FTH1/NCOA4 by artesunate ameliorates salivary gland dysfunction in Sjögren’s Disease

doi: 10.1186/s13020-026-01354-8

Figure Lengend Snippet: Artesunate disrupts iron-ROS coupling to block ferroptosis execution. A , B Confocal microscopy of intracellular ROS and FerroOrange in salivary gland epithelial cells (SGECs) across treatment groups, with relative mean fluorescence intensity (MFI; 20 images from 3 mice/group, n = 3) and 2D intensity histograms showing Pearson’s coefficient (PR), red Manders’ coefficient (M1), and green Manders’ coefficient (M2). Scale = 100 μm. C , D Confocal microscopy of intracellular JC-1 (red/green) in SGECs across treatment groups, with relative MFI, JC-1 red/green ratio, and semi-quantification (MFI; 20 images from 3 cells/group, n = 3). Scale = 40 μm. G Transmission electron microscopy of mitochondrial morphology in SGECs. Data are mean ± SEM. *** p < 0.001, **** p < 0.0001

Article Snippet: Briefly, the sections were subjected to antigen retrieval and treated with a tissue spontaneous fluorescence quencher (G1221, Servicebio).

Techniques: Blocking Assay, Confocal Microscopy, Fluorescence, Transmission Assay, Electron Microscopy